產(chǎn)品詳情
簡(jiǎn)單介紹：
重組大鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白與其它公司提供的重組蛋白不同，rRtFGF-9蛋白產(chǎn)品為采用CFS的無(wú)細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來(lái)的重組蛋白，可表達(dá)出對(duì)細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡(jiǎn)便高效，將蛋白純化過(guò)程中對(duì)蛋白的損傷降低到*小程度.重組大鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白（全長(zhǎng)序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測(cè)、ELISA等試驗(yàn)中.
詳情介紹：
重組大鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白
| Synonyms | GAF, Glia-activating factor, HBGF-9 |
| Species | Rat |
| Accession | P36364 |
| GeneID | 25444 |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白Approximately 23.1 kDa, a single non-glycosylated polypeptide chain containing 205 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | LGEVGSYFGV QDAVPFGNVP VLPVDSPVLL SDHLGQSEAG GLPRGPAVTD LDHLKGILRR RQLYCRTGFH LEIFPNGTIQ GTRKDHSRFG ILEFISIAVG LVSIRGVDSG LYLGMNEKGE LYGSEKLTQE CVFREQFEEN WYNTYSSNLY KHVDTGRRYY VALNKDGTPR EGTRTKRHQK FTHFLPRPVD PDKVPELYKD ILSQS |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組大鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 400 mM NaCl, pH 8.0. |
| Endotoxin | Less than 1 EU/μg of rRtFGF-9-1 as determined by LAL method. |
| Reconstitution | 重組大鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠成纖維細(xì)胞生長(zhǎng)因子9蛋白 | |
| SDS-PAGE |
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| Reference |
1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9. 2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31. 3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94. |
| Background | Fibroblast growth factor-9 (FGF-9) is a member of the fibroblast growth factor (FGF) family. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. FGF-9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. FGF-9 is a monomer and interacts with FGFR1, FGFR2, FGFR3 and FGFR4. The rat FGF-9 shares 98 % a.a. sequence identity with human FGF-9. |
