產品詳情
簡單介紹：
重組恒河猴S100鈣結合蛋白B.蛋白與其它公司提供的重組蛋白不同，rRhS100B蛋白產品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組恒河猴S100鈣結合蛋白B.蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組恒河猴S100鈣結合蛋白B.蛋白
| Synonyms | Protein S100-P, Migration-inducing gene 9 protein, MIG9, Protein S100-E, S100 calcium-binding protein P |
| Species | Rhesus Macaque |
| Accession | F7ANQ9 |
| GeneID | 708117 |
| Source | 重組恒河猴S100鈣結合蛋白B.蛋白Escherichia coli. |
| Molecular Weight | Approximately 10.6 kDa, a single non-glycosylated polypeptide chain containing 91 amino acids |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | SELEKAMVAL IDVFHQYSGR EGDKHKLKKS ELKELINNEL SHFLEEIKEQ EVVDKVMETL DSDGDGECDF QEFMAFVAMV TTACHEFFEH E |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組恒河猴S100鈣結合蛋白B.蛋白Data Not Available. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rRhS100B as determined by LAL method. |
| Reconstitution | 重組恒河猴S100鈣結合蛋白B.蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組恒河猴S100鈣結合蛋白B.蛋白 | |
| Reference |
1. Becker T, Gerke V, Kube E, et al. 1992. Eur J Biochem, 207: 541-7. 2. Sato NandHitomi J. 2002. Anat Rec, 267: 60-9. 3. Namba T, Homan T, Nishimura T, et al. 2009. J Biol Chem, 284: 4158-67. 4. Tothova V, Isola J, Parkkila S, et al. 2011. J Cell Biochem, 112: 3373-84. |
| Background | S100B belongs to the S100 family, which containing 2 EF-hand calcium-binding motifs. In humans, S100B protein is encoded by the S100P gene located at 4q16, but genes that encode other numbers of s100 family proteins are almost located at 1q21 as a cluster. S100B is glial-specific and is expressed primarily by astrocytes. Not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein. |
