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產品名稱：重組人膠質細胞系衍生的神經營養因子蛋白
產品型號：rHuGDNF
產品廠商：KALANG
產品文檔：

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簡單介紹：

重組人膠質細胞系衍生的神經營養因子蛋白與其它公司提供的重組蛋白不同，rHuGDNF蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人膠質細胞系衍生的神經營養因子蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人膠質細胞系衍生的神經營養因子蛋白

Synonyms ATF, ATF-1

Species Human

Accession P39905

GeneID 2668

Source Escherichia coli.

Molecular Weight 重組人膠質細胞系衍生的神經營養因子蛋白Approximately 30.1 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.

Quantity 2μg/10μg/1000μg

AA Sequence SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI

Purity 重組人膠質細胞系衍生的神經營養因子蛋白> 97 % by SDS-PAGE and HPLC analyses.

Biological Activity Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 5.0 × 10⁷ IU/mg.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 重組人膠質細胞系衍生的神經營養因子蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, with 0.05 % Tween-20.

Endotoxin Less than 0.1 EU/μg of rHuGDNF as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人膠質細胞系衍生的神經營養因子蛋白

SDS-PAGE

Reference 1. Wood MD, Kim H, Bilbily A, et al. 2012. Muscle Nerve, 46: 122-4.
2. Stahl K, Mylonakou MN, Skare O, et al. 2011. Brain Res, 1378: 105-18.
3. Cornejo M, Nambi D, Walheim C, et al. 2010. Neurochem Res, 35: 1643-51.
4. Boado RJ, Zhang Y, Wang Y, et al. 2008. Biotechnol Bioeng, 100: 387-96.

Background Glial cell-derived neurotrophic factor is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. It is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha. GDNF (monomer) contains seven conserved cysteine residues, one of which (Cys 101) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration. Rat and human mature GDNF shows 93 % sequence identity and have species cross-reactivity.

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Synonyms	ATF, ATF-1
Species	Human
Accession	P39905
GeneID	2668
Source	Escherichia coli.
Molecular Weight	重組人膠質細胞系衍生的神經營養因子蛋白Approximately 30.1 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains.
Quantity	2μg/10μg/1000μg
AA Sequence	SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI
Purity	重組人膠質細胞系衍生的神經營養因子蛋白> 97 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using rat C6 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 5.0 × 10⁷ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	重組人膠質細胞系衍生的神經營養因子蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4, with 0.05 % Tween-20.
Endotoxin	Less than 0.1 EU/μg of rHuGDNF as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人膠質細胞系衍生的神經營養因子蛋白
SDS-PAGE
Reference	1. Wood MD, Kim H, Bilbily A, et al. 2012. Muscle Nerve, 46: 122-4. 2. Stahl K, Mylonakou MN, Skare O, et al. 2011. Brain Res, 1378: 105-18. 3. Cornejo M, Nambi D, Walheim C, et al. 2010. Neurochem Res, 35: 1643-51. 4. Boado RJ, Zhang Y, Wang Y, et al. 2008. Biotechnol Bioeng, 100: 387-96.
Background	Glial cell-derived neurotrophic factor is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. It is a founding member of the GDNF family of ligands (GFL) and has been shown to interact with GFRA2 and GDNF family receptor alpha. GDNF (monomer) contains seven conserved cysteine residues, one of which (Cys 101) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration. Rat and human mature GDNF shows 93 % sequence identity and have species cross-reactivity.

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產品名稱：重組人膠質細胞系衍生的神經營養因子蛋白