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產品名稱：核因子κ-B的重組人可溶性受體激活劑蛋白
產品型號：rHusRANK Receptor/TNFRSF11A
產品廠商：KALANG
產品文檔：

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簡單介紹：

核因子κ-B的重組人可溶性受體激活劑蛋白與其它公司提供的重組蛋白不同，rHusRANK Receptor/TNFRSF11A蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.核因子κ-B的重組人可溶性受體激活劑蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

核因子κ-B的重組人可溶性受體激活劑蛋白

Species Human

Accession Q9Y6Q6

GeneID 8792

Source Escherichia coli.

Molecular Weight 核因子κ-B的重組人可溶性受體激活劑蛋白Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.

Quantity 20μg/100μg/1000μg

AA Sequence QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK

Purity > 98 % by SDS-PAGE and HPLC analyses.

Biological Activity 核因子κ-B的重組人可溶性受體激活劑蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 核因子κ-B的重組人可溶性受體激活劑蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150mM NaCl.

Endotoxin Less than 0.1 EU/μg of rHusRANK Receptor as determined by LAL method.

Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

核因子κ-B的重組人可溶性受體激活劑蛋白

SDS-PAGE

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Species	Human
Accession	Q9Y6Q6
GeneID	8792
Source	Escherichia coli.
Molecular Weight	核因子κ-B的重組人可溶性受體激活劑蛋白Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.
Quantity	20μg/100μg/1000μg
AA Sequence	QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	核因子κ-B的重組人可溶性受體激活劑蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	核因子κ-B的重組人可溶性受體激活劑蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150mM NaCl.
Endotoxin	Less than 0.1 EU/μg of rHusRANK Receptor as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
核因子κ-B的重組人可溶性受體激活劑蛋白
SDS-PAGE

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產品名稱：核因子κ-B的重組人可溶性受體激活劑蛋白